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NEN Life Science random primer plus extension labeling system
Random Primer Plus Extension Labeling System, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+primer+plus+extension+labeling+system/random+primer+extension+labeling+kit/pm16962266-76-9-15
Average 90 stars, based on 1 article reviews
random primer plus extension labeling system - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

other:

Article Title: Differential effects of progestogens, by type and regimen, on estrogen-metabolizing enzymes in human breast cancer cells.
Article Snippet: Objectives: To investigate the in vitro effects of five progestogens commonly used in hormone replacement therapy (HRT) on estrogen-metabolizing enzymes in human breast cancer cells.. Methods: The human hormone-dependent breast cancer cell lines T47D, MCF-7, and MCF-7aro were cultured with estradiol (E2) and progestogens.. The mRNA levels of estrogen-metabolizing enzymes were determined by RT-PCR or Northern blot, and enzyme activities by radiolabeled substrates.

Labeling:

Article Title: Expression of leptin receptor in human endometrium and fluctuation during the menstrual cycle.
Article Snippet: Aliquots of the DNA products were sequenced by the dye terminator method using a model 100 DNA analyzer (PE Applied Biosystems, Foster City, CA), and the sequences were confirmed to be equal to those reported in the GenBank databank (U43168 and U18915 for OB-RL and ob, respectively). .. The probes were radiolabeled with [a-32P]deoxy-CTP using a Random Primer Plus extension labeling system (NEN Life Science Products, Boston, MA). ..

Article Title: Progesterone induction of 17beta-hydroxysteroid dehydrogenase type 2 during the secretory phase occurs in the endometrium of estrogen-dependent benign diseases but not in normal endometrium.
Article Snippet: Aliquots of the DNA products were sequenced by the dye terminator method using a model 100 DNA analyzer (PE Applied Biosystems, Inc., Foster City, CA) and the sequence for 17bHSD2 was confirmed to be equal to the sequence reported in the Genbank databank. .. The probes were radiolabeled with [a-32P]dCTP using a Random primer plus extension labeling system (NEN Life Science Products, Boston, MA). ..

Article Title: Serotonin-mediated production of interstitial collagenase by uterine smooth muscle cells requires interleukin-1alpha, but not interleukin-1beta.
Article Snippet: .. The blot is baked at 80 °C for 3 h and then prehybridized for 30 min in 4 3 SSC, 50% deionized formamide, 20 mM Tris-HCl (pH 8), 10% dextran sulfate, 200 mg/ml denatured salmon sperm DNA, and 1 3 Denhardt’s solution which consists of 0.02% Ficoll, 0.02% polyvinylpyrrolidone, 0.02% bovine serum albumin. cDNA probes were radiolabeled with [a-32P]dCTP (3000 Ci/mmol) using the Random Primer Plus Extension Labeling System (NEN Life Science products Inc., Boston, MA). .. Autoradiographic signals were quantified by video capture and digital image processing using the BioImage system (BioImage/Millipore, Ann Arbor, MI).



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